TECH OFFER

An Antigenic Recombinant Hypothetical Lipoprotein from Burkholderia Pseudomallei and a Novel Method Thereof

TECHNOLOGY OVERVIEW

The present invention discloses a design and production of a recombinant antigen based on a hypothetical lipoprotein (rHLP) (rBurP16-532) from Burkhoderia pseudomallei and a novel method for synthesizing an antigenic recombinant hypothetical lipoprotein (rHLP) from Burkholderia pseudomallei. The method comprises series of steps:1. construction of recombinant plasmid; 2. Expression and purification of recombinant lipoprotein; 3. analysis of purified recombinant lipoprotein; and 4. confirming the reactivity of the purified recombinant lipoprotein (rHLP). The pTriEx_rBurP16-532 plasmid containing the gene sequence for residues 16-532 of the B. pseudomallei hypothetical lipoprotein (rBurP16-532) cloned into the pTriEx-3 Hygro expression vector, wherein the vector expresses the rHLP protein, consisting the HIG and FLAG tag fused to the N-terminus of the residues 16-532 of the B. pseudomallei hypothetical lipoprotein. The hypothetical lipoprotein (rHLP) (rBurP16-532) is reactive to the antibodies produced in the sera of melioidosis patients and is used for different medical and healthcare purposes mainly as vaccineor diagnostic agent against the disease of melioidosis.

Mega - Trends

Chemicals and Materials

Technology Readiness Level (TRL)

TRL 3

Patent Number

PI 2016704195

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Contact person for this offer:

ChM Dr. Lee Ching Shya, PhD (Dual), RTTP

Technology Transfer Manager

Email: leecs@um.edu.my

Tel: +603-7967-7351/ 013-2250151

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