TECH OFFER

Method of Cloning, Expression, Purification of Serine Protease(S) From Lignosus Rhinocerus

TECHNOLOGY OVERVIEW

Disclosed are a method for cloning genes of a plurality of serine proteases (SPs). The method includes a step of extracting ribonucleic acid (RNA) from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) is obtained from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) include a GME4347 RNA and a GME8711 RNA. The method includes a step of performing a reverse transcription-polymerse chain reaction (RT-PCR) to synthesize a complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA. The method includes a step of cloning the complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA into a pGEM-T Easy Vector System I and transformed into Escherichia coli BL21 (DE3) cells.

Mega - Trends

Healthcare

Technology Readiness Level (TRL)

TRL 3

Patent Number

PI 2020003670

Get the technology fact sheet here:

Contact person for this offer:

ChM Dr. Lee Ching Shya, PhD (Dual), RTTP

Technology Transfer Manager

Email: leecs@um.edu.my

Tel: +603-7967-7351/ 013-2250151

MAKE AN ENQUIRY

press to zoom

press to zoom
1/1

MORE INFORMATION

You have a question to know about technologies or cooperations? 
Please Contact Us:

+603 - 7967 7351 / 013-2250151