
TECH OFFER
Method of Cloning, Expression, Purification of Serine Protease(S) From Lignosus Rhinocerus
TECHNOLOGY OVERVIEW
Disclosed are a method for cloning genes of a plurality of serine proteases (SPs). The method includes a step of extracting ribonucleic acid (RNA) from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) is obtained from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) include a GME4347 RNA and a GME8711 RNA. The method includes a step of performing a reverse transcription-polymerse chain reaction (RT-PCR) to synthesize a complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA. The method includes a step of cloning the complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA into a pGEM-T Easy Vector System I and transformed into Escherichia coli BL21 (DE3) cells.
Contact person for this offer:
ChM Dr. Lee Ching Shya, PhD (Dual), RTTP
Technology Transfer Manager
Email: leecs@um.edu.my
Tel: +603-7967-7351/ 013-2250151


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