TECH OFFER

Primers for Detection of Zika Viruses Using Single-Tube Reverse Transcription Loop-Mediated Isothermal Amplification (RT-Lamp)

TECHNOLOGY OVERVIEW

The present invention discloses a single-tube reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for early and rapid detection of Zika virus (ZIKV) infection. The single-tube RT-LAMP assay disclosed according to the present invention comprises of a combination of 6 primer sequences or 6 nucleotide fragments represented by sequences (SEQ ID NO: 1) to (SEQ ID NO: 6) for isothermal detection of ZIKV RNA. In another embodiment, the single-tube RTLAMP assay developed in the present invention is specific and sensitive for broad coverage detection of both the Asian and African ZIKV strains. The RT-LAMP assay can greatly enhance diagnosis of Zika in rural clinics and healthcare facilities where resources are limited.

Mega - Trends

Healthcare

Technology Readiness Level (TRL)

TRL 4

Patent Number

PI 2020004241

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Contact person for this offer:

ChM Dr. Lee Ching Shya, PhD (Dual), RTTP

Technology Transfer Manager

Email: leecs@um.edu.my

Tel: +603-7967-7351/ 013-2250151

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