TECH OFFER

Real-Time Polymerase Chain Reaction Procedure For Detecting And Quantification Of Cytomegalovirus

TECHNOLOGY OVERVIEW

A method for detecting presence of Cytomegalovirus in a biological sample comprises the steps of isolating DNA sample from the biological sample; mixing an effective amount of the isolated DNA sample into a reaction reagent containing fluorescent moieties attached oligonucleotides probe SEQ. No 1 and SEQ. No 2 to form a mixture; subjecting the mixture for thermo cycling of 30 to 45 cycles including denaturation at 90 to 95°C for 25 to 35 second followed by annealing at 58 to 62°C for 35 to 45 second then extension at 66 to 74°C for 30 to 90 second to produce amplicons derived from Cytomegalovirus; verifying presence of Cytomegalovirus in the biological sample via detection of the produced amplicons in the mixture using melting curve analysis.

Mega - Trends

Healthcare, Wellness and Well Being (HWW)

Technology Readiness Level (TRL)

TRL 3

Patent Number

MY-176010-A

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Contact person for this offer:

ChM Dr. Lee Ching Shya, PhD (Dual), RTTP

Technology Transfer Manager

Email: leecs@um.edu.my

Tel: +603-7967-7351/ 013-2250151

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