Method of Cloning, Expression, Purification of Serine Protease(S) From Lignosus Rhinocerus

Disclosed are a method for cloning genes of a plurality of serine proteases (SPs). The method includes a step of extracting ribonucleic acid (RNA) from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) is obtained from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) include a GME4347 RNA and a GME8711 RNA. The method includes a step of performing a reverse transcription-polymerse chain reaction (RT-PCR) to synthesize a complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA. The method includes a step of cloning the complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA into a pGEM-T Easy Vector System I and transformed into Escherichia coli BL21 (DE3) cells.