TECH OFFER
Real-Time Viral Detection Method and Kit For Infectious Hypodermal and Haematopoietic Necrosis Virus
TECHNOLOGY OVERVIEW
A method for quantitatively detecting infectious hypodermal and haematopoietic necrosis virus (IHHNV) in a biological sample, comprising the steps of obtaining a primer pair having nucleotide sequences set forth in SEQ ID NO: 1 and SEQ ID NO: 2, and a fluorescent probe having a nucleotide sequence set forth in SEQ ID NO: 3; amplifying a non-structural protein coding region of the virus in the biological sample by a real-time polymerase chain reaction (PCR) using the primer pair and the fluorescent probe; and determining the presence and quantity of the virus in the biological sample with reference to a positive control plasmid.
Contact person for this offer:
ChM Dr. Lee Ching Shya, PhD (Dual), RTTP
Technology Transfer Manager
Email: leecs@um.edu.my
Tel: +603-7967-7351/ 013-2250151